The hemoglobin phenotypes and the gene frequencies of 223 Cheju native horses were studied by starch gel electrophoresis. The results obtained were as follows:
1. In the hemoglobin phenotypes, three phenotypes. HbAA, HbAa and Hbaa, which were controlled by two allelic genes, Hb^A and Hb^a, were observed and their frequencies of appearance were 65.47%, 30.04% and 4.48% respectively.
2. The distribution of gene frequency was calculated as 0.805 in Hb^A and 0.195 in Hb^a.
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